Enzyme dosage calculator.
Four quick tools for formulators: standardise a concentrate to a target activity, calculate process dosage, plan probiotic counts with overage, and convert activity units.
Standardise a concentrate to a target activity
How much high-activity concentrate and how much carrier do you need to make a batch at your target activity?
- Dilution
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- Concentrate share
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–Enzyme dosage for your process
Enter the activity your process needs per kg of substrate — from your trials or our application guidance.
- Dose rate
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- As % of substrate
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- ppm
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–Probiotic culture per batch
How much probiotic powder do you need for your label claim per serving, including overage?
- Per serving
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- Servings per batch
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- Culture share
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–Activity unit converter
Convert between per-gram and per-milligram activity, units and katal, and probiotic counts.
1 U = 1 µmol/min ≈ 16.67 nkat · 1 U/mg = 1,000 U/g · 1 BN = 10⁹ CFU/g
Indicative calculations only. Actual dosage depends on substrate, pH, temperature, time and the result you need — confirm with lab or pilot trials. Ask our application team for a starting dose and a sample.
The formulas, explained.
Every result shows its formula, so you can check it or reuse it in your own batch sheets. New to the units? See the enzyme glossary.
Multiply the target activity by the batch size and divide by the activity of the concentrate: concentrate (kg) = target activity × batch (kg) ÷ concentrate activity. The rest of the batch is carrier. For example, 100 kg at 10,000 U/g from a 100,000 U/g concentrate needs 10 kg concentrate and 90 kg carrier.
Decide how many activity units you need per kilogram of substrate (from the supplier’s guidance or your trials), multiply by the substrate quantity, and divide by the product activity in units per gram. The result is grams of enzyme product per batch.
Some activity or viable count can be lost during mixing, heat processing and storage. An overage — extra material added at manufacture — makes sure the label value is still met at the end of shelf life. The right overage depends on the product and process and is confirmed by stability data.
Only if they use the same assay. Units such as U, XU or CMC U depend on the substrate, pH, temperature and method used. When methods differ, compare products in a side-by-side application trial.
No. The calculator does the arithmetic only. Optimum dosage depends on your substrate, process conditions and target result, and should be confirmed with lab or pilot trials. Our application team can suggest a starting point.
Need a starting dose?
Tell us your substrate, process conditions and target result — we’ll recommend a product, a starting dosage and send a sample with its TDS.